Modification of soil environment by different farming practices can si
gnificantly affect crop growth. Tillage causes soil disturbance, alter
ing the vertical distribution of soil organic matter and plant nutrien
t supplies in the soil surface, and it may affect the enzyme activity
and microbial biomass which are responsible for transformation and cyc
ling of organic matter and plant nutrients. In this study, the influen
ce of three conventional tillage systems (shallow plowing, deep plowin
g and scarification) at different depths on the distribution and activ
ity of enzymes, microbial biomass and nucleic acids in a cropped soil
was investigated. Analysis of variance for depth and tillage showed th
e influence of the different tillage practices on the activity of some
enzymes and on the nucleic acids. Glucosidase, galactosidase, nitrate
reductase and de hydrogenase activity were significantly affected by
the three tillage modalities. Activity in the upper layer (0-20 cm) wa
s higher in the plots tilled by shallow plowing and scarification than
in those tilled by deep plowing. Positive relationships were observed
between the soil enzymes themselves, with the exception of urease and
pyrophosphatase activity. Moreover, significant correlations were fou
nd between DNA and beta-galactosidase, and between RNA and beta-glucos
idase, beta-galactosidase, alkaline phosphatase and phosphodiesterase.
alpha-Glucosidase, beta-galactosidase, alkaline phosphatase and phosp
hodiesterase were highly correlated with biomass C determined by the f
umigation-extraction method.