M. Huleihel et al., Involvement of serum and lipopolysaccharide in the production of interleukin-1-and interleukin-6-like molecules by human sperm cells, AM J REPROD, 43(1), 2000, pp. 41-46
PROBLEM: To examine the capacity of sperm cells from fertile and infertile
men to secrete interleukin (IL)-6, and the involvement of serum factors and
lipopolysaccharide (LPS) in the regulation of IL-6 and IL-I production by
sperm cells.
METHODS: Swim-up sperm cells from fertile (donors) and oligoteratoasthenosp
ermic (OTA)-infertile men were incubated with or without 5% fetal calf seru
m (FCS) and LPS (10 mu g/mL) for 2-24 hr. After incubation, IL-6 and IL-1 b
ioactivities were measured in supernatants and lysates by specific bioassay
s (B9 cell proliferation assay and 1A-5 system, respectively).
RESULTS: 1L-6- and IL-1-like activities were observed to be produced by swi
m-up sperm cells from both study groups. Stimulation of swim-up sperm cells
with either LPS or FCS or both together did not affect their capacity to p
roduce IL-1. However, LPS, but not serum increased the secretion levels of
IL-6 by swim-up sperm cells.
CONCLUSIONS: Swim-up sperm cells from both study groups constitutively prod
uce IL-6 and IL-1, and serum components did not affect this capacity. Howev
er, LPS was shown to increase the capacity of swim-up sperm cells of both s
tudy groups to secrete IL-6, but not IL-1. Cytokines may be involved in the
physiology and pathophysiology of sperm functions and, thus, may affect ma
le fertility.