ATP ADP ANTIPORTER-MEDIATED AND ASPARTATE/GLUTAMATE ANTIPORTER-MEDIATED FATTY ACID-INDUCED UNCOUPLING OF LIVER-MITOCHONDRIA IN INCUBATION MEDIA DIFFERING IN ION COMPOSITION/

Substitution of potassium cloride for sucrose in the incubation medium of liver mitochondria or addition of magnesium cloride to the sucrose medium produces a small (if any) effect on the palmitate-induced unco upling and its sensitivity to carboxyatractylate and aspartate, but it exerts a pronounced increase in the recoupling effect of glutamate on this uncoupling. 20 - 40 mu M cetyltrimethyl ammonium bromide increas es the recoupling effect of glutamate and aspartate but decreases that of carboxyatractylate. The data are in line with suggestion that both ATP/ADP antiporter and aspartate/glutamate antiporters are involved i n the fatty acid-induced uncoupling.