K. Yamamoto et al., Highly-sensitive flow injection determination of hydrogen peroxide with a peroxidase-immobilized electrode and its application to clinical chemistry, ANALYT CHIM, 406(2), 2000, pp. 201-207
A flow injection system for determination of hydrogen peroxide was construc
ted using a peroxidase/ferrocene-embedded carbon paste (POD-Fc-CP) electrod
e covered with a membrane. H2O2 was reduced bioelectrocatalytically at 100
mV (versus Ag/AgCl/sat. KCl). A Nafion(R)-coated cellulose acetate membrane
prepared on the surface of water proved to be suitable for the covering me
mbrane. The detection limit (signal :noise = 6) for H2O2 was 200 fmol (10 n
M x 20 mu l) and the response linearity was retained at least up to 25 mu M
. The relative standard deviation was 4.4% for 1.0 mu M H2O (n = 7). The fl
ow injection system was successfully applied to the determination of glucos
e and urate in serum by using the corresponding oxidase-immobilized pre-col
umn. The detection limits were 1.5 pmol for glucose and 2 pmol for urate. (
C) 2000 Elsevier Science B.V. All rights reserved.