Studies on the fluorescence reaction between nucleic acid and the complex of cobalt(II) with 5-(3-fluoro-4-chlorophenylazo)-8-sulfonamidoquinoline and its applications
Qe. Cao et al., Studies on the fluorescence reaction between nucleic acid and the complex of cobalt(II) with 5-(3-fluoro-4-chlorophenylazo)-8-sulfonamidoquinoline and its applications, ANALYT BIOC, 277(2), 2000, pp. 214-220
Based on the enhancement of the fluorescence intensity of the complex of 5-
(3-fluoro-4-chlorophenylazo)-8-sulfonamidoquinoline (FCPBSQ) with Co2+ by n
ucleic acid in the presence of Tween 80 and in the weakly basic medium, a f
luorescence method for the determination of nucleic acid was proposed, The
calibration graphs for the determination of denatured calf thymus DNA (ct D
NA), fish sperm DNA (fs DNA), and yeast RNA (yt RNA) were obtained in conce
ntration ranges 0.050-4.0, 0.10-3.0, and 0.050-3.0 mu g/ml with limits of d
etection of 0.010, 0.030, and 0.020 mu g/ml, respectively, The method has b
een satisfactorily used for the determination of DNA in wheat cell extracti
on and ct DNA, fs DNA, and yt RNA in synthetic samples. Investigations on t
he binding mode by the Scatchard plots method suggested that both intercala
tion and electrostatic binding modes existed in this system. (C) 2000 Acade
mic Press.