Subtractive hybridization and differential screening identified two genes differentially expressed after induction of in vitro (atypical) terminal differentiation in the NSCLC-N6 cell line by a marine substance (bistramide K)

Non-small-cell lung carcinoma is generally refractory to chemotherapy. The difficulties that arise in the treatment of this type of tumor make it nece ssary to develop new therapeutic strategies. Previous work done in our labo ratory showed that a marine substance named bistramide K induced in vitro ( atypical) terminal differentiation of NSCLC-N6 cell line. This activity is linked to a growth arrest of NSCLC-N6 cell line and an irreversible block a t the GI phase of the cell cycle (G1DT). In order to identify the genes tha t could be expressed after the treatment by the drug, we constructed a subt ractive cDNA library with enriched mRNA extracted from BK-treated NSCLC-N6. After differential hybridization and DNA sequencing, We identified two seq uences. The sequence identified Sol the clone 8 showed strong homology to t he sequence of the ribosomal protein L35A. The sequence identified Soi the clone 4 did nor show any homology with known sequences in official gene dat a banks.