H. Ishida et al., Allogeneic class I major histocompatibility complex gene transfer in murine neuroblastoma in vivo, ANTICANC R, 19(6B), 1999, pp. 5367-5373
In vivo intratumoral gene transfer of allogeneic class I major histocompati
bility complex (MHC) genes augments the immune response against weak tumor
antigens. In this study, mice inoculated with the allogeneic MHC mollecule
(H-2K(b)), had transduced-murine neuroblastoma C1300S3 cells showed prolong
ed survival relative to non-transduced or neo transduced tumors (p<0.005).
Interestingly, direct in vivo gene transfer of H-2K(b) plasmid DNA complexe
d with HVJ-liposomes into S3 tumors was highly efficient resulting in trans
duction of 8% of the interstitial cells within the tumor but rarely within
tumor cells, Regression of established tumors and prolonged survival occurr
ed in 50% of mice injected with H-2K(b) in contrast to no tumor I egression
in mice receiving control plasmid (p<0.005). This Study concludes that int
erstitial cells could serve as an important target of intratumoral gene tra
nsfer; and further that HVJ-liposome complexes could be a vehicle for in vi
vo gene transfer.