Rp. Murphy et al., Prospective evaluation of the risk conferred by factor V Leiden and thermolabile methylenetetrahydrofolate reductase polymorphisms in pregnancy, ART THROM V, 20(1), 2000, pp. 266-270
Factor V (FV) Leiden and thermolabile methylenetetrahydrofolate reductase (
MTHFR) are 2 common polymorphisms that have been implicated in vascular thr
ombosis. We determined whether these mutations predicted an adverse outcome
in pregnancy. Second, we looked for an interaction between these 2 mutatio
ns in patients with recurrent fetal loss or thrombosis in pregnancy. Primig
ravid subjects at their booking visit to the National Maternity Hospital (H
olles Street, Dublin, Ireland) were screened for the polymorphisms. Thermol
abile MTHFR and FV Leiden genotypes were detected by either restriction fra
gment length polymorphism or heteroduplex capillary chromatography. The car
rier frequency of FV Leiden in the screened primigravid population was 2.7%
(allele frequency 1.36%), all being heterozygous for the mutation. This va
lue was lower than expected from previous studies in European populations,
Forty-nine percent of the screened population (289 of 584) were heterozygou
s for thermolabile MTHFR, and 10.6% were homozygous (62 of 584). The freque
ncy of the 2 polymorphisms was no higher in those who subsequently develope
d preeclampsia (n=12) or intrauterine growth retardation (n=9), and none of
the screened population developed thrombosis. However, the frequency of FV
Leiden was higher in patients who subsequently miscarried after the first
trimester of pregnancy (allele frequency of 5.5%, P=0.0356), Among those po
sitive for FV Leiden, 3 of 27 miscarried, compared with 24 of 572 of FV Lei
den-negative patients (11% versus 4.2%). No interaction was found between t
he 2 mutations in the control or patient populations. In patients with a pr
ior history of venous thrombosis, the carrier rate of FV Leiden was increas
ed (4 of 33, allele frequency of 7.6%, P=0.0115), In contrast, the carrier
frequency for thermolabile MTHFR was no higher, and there was no interactio
n between the 3 mutations. Neither mutation occurred at a significantly hig
her frequency in patients with a prior history of recurrent fetal loss. In
conclusion, FV Leiden is a risk factor for thrombosis in pregnancy and poss
ibly for second-trimester miscarriage independent of thermolabile MTHFR. Ho
wever, prospective analysis suggests that the risk conferred by FV Leiden i
s low in a primigravid population. The thermolabile MTHFR genotype was not
implicated in any adverse outcome.