cDNA cloning and chromosomal mapping of the gene encoding adenylate kinase2 from Drosophila melanogaster

As a step toward understanding of the role of adenylate kinase (AK) in ener gy metabolism, we analyzed this enzyme in Drosophila melanogaster. The enzy me activities of all three AK isozymes were determined in cell-free extract s of flies, and their proteins were detected by Western blot analysis using polyclonal antibodies against the mammalian isozymes. A cDNA encoding aden ylate kinase was isolated from D, melanogaster cDNA library. The cDNA encod es a 240-amino acid protein, which shows high similarity to bovine, human a nd rat AK2, and hence was named DAK2. Preliminary subcellular fractionation analysis indicated that DAK2 is localized in both cytoplasm and mitochondr ia. In situ hybridization to salivary gland polytene chromosomes revealed t hat the Dak2 gene is located at 60B on the right arm of the second chromoso me. (C) 2000 Elsevier Science B.V. All rights reserved.