Characterization of three new manganese peroxidase genes from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Three new genes (Cs-mnp2A, Cs-mnp2B and Cs-mnp3) coding for manganese-depen dent peroxidase (MnP) have been identified in the white-rot basidiomycete C eriporiopsis subvermispora. The mature proteins contain 366 (MnP2A and MnP2 B) and 364 (MnP3) amino acids, which are preceded by leader sequences of 21 and 24 amino acids, respectively. Cs-mnp2A and Cs-mnp2B appear to be allel es, since the corresponding protein sequences differ in only five residues, The upstream region of Cs-mnp2B contains a TATA box, AP-1 and AP-2 sites, as well as sites for transcription regulation by metals (two), cAMP (two) a nd xenobiotics (one). Some of these elements are also found in the regulato ry region of Cs-MnP3, Transcription of Cs-mnp2A and Cs-mnp2B, but not that of Cs-mnp3, is activated by manganese. (C) 2000 Elsevier Science B.V. All r ights reserved.