Cholesterol crystalline polymorphism and the solubility of cholesterol in phosphatidylserine

Citation
Rm. Epand et al., Cholesterol crystalline polymorphism and the solubility of cholesterol in phosphatidylserine, BIOPHYS J, 78(2), 2000, pp. 866-873
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOPHYSICAL JOURNAL
ISSN journal
00063495 → ACNP
Volume
78
Issue
2
Year of publication
2000
Pages
866 - 873
Database
ISI
SICI code
0006-3495(200002)78:2<866:CCPATS>2.0.ZU;2-B
Abstract
There is a marked hysteresis between the heating and cooling polymorphic ph ase transition of anhydrous cholesterol. At a scan rate of 0.05 degrees C/m in the difference in transition temperatures between heating and cooling sc ans is similar to 10 degrees C, This phenomenon also occurs with mixtures o f cholesterol with phosphatidylserine and can result in an underestimation of the amount of crystalline cholesterol in a sample that has not been cool ed sufficiently. With 1-palmitoyl-2-oleoyl phosphatidylserine and 1-stearoy l-2-oleoyl phosphatidylserine the cholesterol crystallites form while the l ipid remains in the L-alpha phase. Sonication of dimyristoyl phosphatidylse rine with a 0.4 mol fraction cholesterol results in the loss of cholesterol crystallite diffraction, but only a partial loss of the polymorphic transi tion detected by calorimetry. We therefore conclude that the thermal histor y of the sample can have profound effects on the appearance of the polymorp hic phase transition of cholesterol by differential scanning calorimetry. D epending on the morphology of the vesicles, diffraction methods may underev aluate the amount of cholesterol crystallites present.