Mechanism of antioxidative activity of fluvastatin-determination of the active position

In order to clarify the mechanism of action for the antioxidative activity of fluvastatin sodium (FLV, (+/-)sodium (3RS, 5RS, 6E)-7-[3-(4-fluorophenyl )-1-(1-methylethyl)-1H-indol-2-yl]-3,5-dihydroxy-6-heptanoate) and its deri vatives, reaction of the corresponding methyl ester of FLV with di-tert-but yl diperoxyoxalate was examined, and the corresponding keto derivative was isolated from the reaction mixture. On the basis of this result, it was con cluded that the active site is the allylic carbon conjugated with the indol e ring.