Microtubules have been shown to alter contraction in cardiac myocytes throu
gh changes in cellular stiffness. However, an effect on excitation-contract
ion coupling has not been examined. Here we analyze the effects of microtub
ule disruption by 1 mu mol/L colchicine on calcium currents (I-Ca) and [Ca2
+](i) transients in rat ventricular myocytes. I-Ca was studied using the wh
ole-cell patch-clamp technique. Colchicine treatment increased I-Ca density
(peak values, -4.6+/-0.4 and -9.1+/-1.3 pA/pF in 11 control and 12 colchic
ine-treated myocytes, respectively; P<0.05). I-Ca inactivation was well fit
ted by a biexponential function. The slow component of inactivation was unc
hanged, whereas the fast component was accelerated after colchicine treatme
nt (at -10 mV, 11.8+/-1.0 versus 6.7+/-1.0 ms in control versus colchicine-
treated cells; P<0.005). [Ca2+](i) transients were analyzed by flue-3 epifl
uorescence simultaneously with I-Ca. Peak [Ca2+](i) transients were signifi
cantly increased in cardiac myocytes treated with colchicine. The values of
F/F-0 at 0 mV were 1.1+/-0.02 in 9 control cells and 1.4+/-0.1 in 11 colch
icine-treated cells (P<0.05). beta-Adrenergic stimulation with 1 mu mol/L i
soproterenol increased both I-Ca and [Ca2+](i) transient in control cells.
However, no significant change was induced by isoproterenol on colchicine-t
reated cells. Colchicine and isoproterenol effects were similar and not add
itive. Inhibition of adenylyl cyclase by 200 mu mol/L 2'-deoxyadenosine 3'-
monophosphate blunted the colchicine effect. We suggest that beta-adrenergi
c stimulation and microtubule disruption share a common pathway to enhance
I-Ca and [Ca2+](i) transient.