Histopathology and cytopathology of white spot syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation

Citation
Yg. Wang et al., Histopathology and cytopathology of white spot syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation, DIS AQU ORG, 39(1), 1999, pp. 1-11
Citations number
26
Categorie Soggetti
Aquatic Sciences
Journal title
DISEASES OF AQUATIC ORGANISMS
ISSN journal
01775103 → ACNP
Volume
39
Issue
1
Year of publication
1999
Pages
1 - 11
Database
ISI
SICI code
0177-5103(199912)39:1<1:HACOWS>2.0.ZU;2-A
Abstract
Since 1994, white spot syndrome virus (WSSV) has been detected in cultured shrimp Penaeus monodon in Peninsular Malaysia. The gross signs, target orga ns and histo-cytopathology for the viral infection were studied and it was found to infect most organs and tissues including oocytes, but not hepatopa ncreatocytes and epithelial cells of the midgut, which were regarded as ref ractory tissues. Based on a time-sequence of ultrastructural cytopathology, 4 cytopathic profiles and 6 phases of viral morphogenesis were described. The virions were elliptical to short rods with trilamilar envelopes that me asured 305 +/- 30 x 127 +/- 11 nm. Viral nucleosomes were often present sin gly in infected nuclei and were associated with the early stages of viral r eplication. The structure of WSSV pathognomonic white, cuticular lesions wa s examined at the microscopic and ultrastructural levels and the mechanism of their formation appeared to be related to the disruption of exudate tran sfer from epithelial cells to the cuticle via cuticular pore canals.