Expression of a PKR dominant-negative mutant in myogenic cells interferes with the myogenic process

In this study, we explored the possibility that PKR, a dsRNA-activated regu latory protein, is an essential component in the differentiation program of myogenic cells in vitro. For this purpose, we used a retroviral expression vector pMV7, harboring the PKR dominant-negative mutant PKR Delta 6 (pMV7- p68 Delta 6). Murine C2C12 myogenic cells were transfected either with pMV7 or with pMV7-p68 Delta 6. Neomycin-resistant clones from both types were i solated and expanded and the results obtained with the representative clone s C2-NEO (transfected with pMV7) and clone 17 and clone 22 (both transfecte d with pMV7-p68 Delta 6) are presented. In clone 17 and 22 cells, regardles s of IFN treatment, a similar level of the transfected human p68 PKR mutant was detected. This protein was absent in CS-NEO cells. In parallel, in all types of cells, a low basal level of the endogenous murine p65 PKR protein was observed, which was further induced by IFN. However, PKR enzymatic act ivity was significantly induced by IFN only in C2-NEO cells, while it was h ardly detected in both clones 17 and 22, even after IFN treatment. Furtherm ore, in contrast to C2-NEO cells, only a slight to moderate increase in enz ymatic activity was observed in clone 17 and 22 differentiating cells. Next , cells were grown either in growth medium (GM) or differentiation medium ( DM), and the progression of the myogenic program was studied. An inhibition in myotube formation in clone 17 versus C2-NEO cells cultivated in DM was clearly observed. Furthermore, while the growth rate and thymidine incorpor ation were reduced in C2-NEO cells grown in DM, both clone 17 and 22 cells were less affected under the same conditions. Similarly, a delay in the acc umulation of the transcription factors MyoD and myogenin, as well as in cre atine kinase activity and accumulation of troponin T, was detected in DM-cu ltivated clone 17 and clone 22 cells. Moreover, a delay in the induction of p21 (WAF1), in down-regulation of cyclin D1 and cmyc, and in the accumulat ion of the underphosphorylated form of pRb was also observed in clone 17 ce lls. We conclude that inhibition of endogenous PKR activity by a PKR domina nt-negative mutant interferes with the myogenic program of murine C2C12 myo genic cells. (C) 2000 Academic Press.