Genomic structure and chromosomal localization of the rat protein kinase Cdelta-gene

Protein kinase C delta (PKC delta) is a widely expressed calcium-independen t PKC isozyme that is induced at mRNA and protein levels upon stimulation o f different cellular pathways. We found the rat PKC delta gene to consist o f 19 exons and to span approximately 29 kb. The exon-intron junctions follo w the GT/AG rule. The 5' untranslated region is nearly 12 kb in length, and the transcription initiation site is surrounded by CG-rich sequences. The 5' flanking region contains putative binding sites for activator protein 1 (AP-1), nuclear factor kappa B (NF kappa B), stimulatory protein-1 (Sp-1) a nd nerve growth factor induced-C (NGFI-C) transcription factors. The PKC de lta gene is localized at the rat chromosome 19p14. The cloned gene will hel p to elucidate the role of PKC delta in growth, differentation and death of mammalian cells. (C) 2000 Elsevier Science B.V. All rights reserved.