Dynamic capacity studies of CIM (Convective Interaction Media)(R) monolithic columns

The characterization of CIM(R) DEAE monolithic columns in terms of dynamic binding capacity is presented in this paper. Breakthrough experiments were performed for capacity determination. Bovine serum albumin (BSA) was used a s a model protein. It is shown that CIM(R) monolithic columns hale good bat ch-to-batch reproducibility as well as long-term stability. The experiments performed under different linear velocities demonstrated that the dynamic capacity is unaffected at least up to a linear velocity of 2450 cm/h. Furth ermore, the breakthrough curve slope is constant, indicating that the capac ity mould remain constant at even higher linear velocities. The adsorption isotherm of BSA dissolved in 20 mM Tris-HCl buffer shows a constant capacit y of around 30 mg/mL of support down to a concentration of 20 mu g/mL. The capacity is substantially influenced by the ionic strength; however, 20% of the maximal capacity is still preserved at 0.3 M NaCl.