Capillary electrochromatography with monolithic poly(styrene-co-divinylbenzene-co-methacrylic acid) as the stationary phase

A new kind of monolithic capillary electrochromatography column with poly(s tyrene-co-divinylbenzene-co-methacrylic acid) as the stationary phase has b een developed. The stationary phase was found to be porous by scanning elec tron microscopy and the composition of the continuous bed was proved by IR spectroscopy to be the ternary polymer of styrene, divinylbenzene, and meth acrylic acid. The effects of operating parameters, such as voltage, electro lyte, and organic modifier concentration in the mobile phase on electroosmo tic flow were studied systematically, The retention mechanism of neutral so lutes on such a column proved to be similar to that of reversed-phase high performance liquid chromatography. In addition, fast analyses of phenols, c hlorobenzenes, anilines, isomeric compounds of phenylenediamine and alkylbe nzenes within 4.5 min were achieved.