A new monolithic-type HPLC column for fast separations

The application of a new silica-based, monolithic-type HPLC-column for fast separations is presented. The column is prepared according to a new sol-ge l process, which is based on the hydrolysis and polycondensation of alkosys ilanes in the presence of water soluble polymers. The method lends to "rods " made of a single piece of porous silica with a defined pore structure, i. e. macro- and mesopores. The main feature of silica rod columns is a higher total porosity, about 15% higher than of conventional particulate HPLC col umns. The resulting column pressure drop is therefore much lower, allowing operation at higher flow rates including flow gradients. Consequently, HPLC analysis can be performed much faster, as it is demonstrated by various ap plications.