Histopathologic analysis of chromosome aneuploidy in ductal carcinoma in situ

Citation
D. Visscher et al., Histopathologic analysis of chromosome aneuploidy in ductal carcinoma in situ, HUMAN PATH, 31(2), 2000, pp. 201-207
Citations number
29
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
HUMAN PATHOLOGY
ISSN journal
00468177 → ACNP
Volume
31
Issue
2
Year of publication
2000
Pages
201 - 207
Database
ISI
SICI code
0046-8177(200002)31:2<201:HAOCAI>2.0.ZU;2-I
Abstract
Formalin-fixed, paraffin-embedded sections from 28 cases of ductal carcinom a in situ (DCIS; 12 with co-existing invasive neoplasm) were analyzed for n umerical alterations of chromosomes 7, 8, 16, and 17 by performing fluoresc ence in situ hybridization (FISH) using centromeric (alpha-satellite) probe s. Based on signal counts in 200 to 300 nuclei, each hybridization was clas sified as disomic (copy loss in <40%, copy gain in <10%), monosomic (copy l oss in at least 50% of nuclei, partial if 40% to 49%) or trisomic/polysomic (copy gain in at least 20% of nuclei, partial if 10% to 19%). Grade I lesi ons were characterized by complete lack of significant chromosome gain, but 29% showed partial (focal) monosomy. Grade III lesions, in contrast, showe d partial or complete trisomy/polysomy in 88% of hybridizations versus mono somy in only 4%. Grade II DCIS exhibited a mixed pattern of chromosome aneu ploidy: 38% hybridizations were disomic, 36% trisomic/polysomic, and 26% mo nosomic (8 of 10 hybridizations showing complete monosomy occurred in grade II. lesions). Disomic hybridizations exhibiting rare cells (5% to 10%) wit h copy gain were more frequent in tumors with coexisting invasive neoplasm (5 of 17 v 2 of 33, P = .02). In morphologically heterogeneous lesions, hig her-grade foci were characterized by chromosome copy gain relative to corre sponding lower-grade areas in 17 of 22 (77%) hybridizations. These results show the presence of multiple (at least 3) distinct chromosome aneuploidy p atterns in DCIS, in keeping with divergent mechanisms of genetic alteration . Degree of chromosomal instability, moreover, may correlate with progressi on of DCIS to invasive growth, implying that genetic instability is a param eter that impacts the likelihood of early breast carcinoma progression. Cop yright (C) 2000 by W.B. Saunders Company.