Identification of genes regulated by UV/salicylic acid

Purpose: Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The pre sumed effector molecule responsible for this modulation is NF-KB. In the ex periments described here, differential-display RT-PCR was used to identify those cDNAs that are differentially modulated by UV radiation with and with out the addition of salicylic acid. Materials and methods: Differential-display RT-PCR was used to identify dif ferentially expressed genes. Results: Eight such cDNAs are presented: lactate dehydrogenase (LDH-beta), nuclear encoded mitochondrial NADH ubiquinone reductase 24kDa (NDUFV2), elo ngation initiation factor 4B (eIF4B), nuclear dots protein SP100, nuclear e ncoded mitochondrial ATPase inhibitor (IF1), a cDNA similar to a subunit of yeast CCAAT transcription factor HAP5, and two expressed sequence tags (AA 187906 and AA513156). Conclusions: Sequences of four of these genes contained NF-kappa B DNA bind ing sites of the type that may attract transrepressor p55/p55 NF-kappa B ho modimers. Down-regulation of these genes upon UV irradiation may contribute to increased cell survival via suppression of p53 independent apoptosis.