R. Paredes et al., High-dose saquinavir plus ritonavir: Long-term efficacy in HIV-positive protease inhibitor-experienced patients and predictors of virologic response, J ACQ IMM D, 22(2), 1999, pp. 132-138
The year-long antiviral efficacy of a high-dose salvage regimen consisting
of saquinavir (800 mg twice daily) plus ritonavir (400 mg twice daily) was
evaluated in 58 HIV-positive patients who had seen no improvement under fir
st-line protease inhibitor-containing regimens, nor in baseline predictors
of virologic response. The efficacy of therapy was determined by CD4(+)/CD8
(+) and HIV-1 RNA values. The primary endpoint of our study was the percent
age of patients with HIV-1 RNA levels <200 copies/ml (virologic success) at
6 and 12 months of of follow-up. Secondary endpoints were log(10) reductio
n in HIV-1 RNA levels and CD4(+) increases through follow-up. Surrogate mar
kers related with a lower HIV-1 RNA area under the curve were identified at
baseline. Kaplan-Meier analysis and Cox proportional hazards models were a
pplied to identify baseline predictors of achieving viral suppression at <2
00 copies/ml. All analyses were intention to treat-last observation carried
forward. Patients achieved a median HIV-1 RNA level reduction of >0.5 log
through 1 year (-0.59 log,, at 12 months), as well as CD4(+) counts increas
ed significantly (89 cells/mm(3) at 12 months). Overall, 53% of patients we
re likely to achieve HIV-1 RNA levels <200 copies/ml at 6 months. Seventy-s
ix percent of patients who started therapy at HIV-1 RNA levels <5000 copies
/ml but only 42% with baseline viral load of 5000 to 30000 copies/ml and 18
.7% with baseline viral load >30,000 copies/ml were likely to achieve viral
suppression at 6 months (p < .001, log-rank test). Patients with baseline
HIV-1 RNA levels between 5000 and 30,000 copies/ml (relative hazard [RH], 0
.39; 95% confidence interval [CI], 0.01 to 0.98; p = .0396) and patients wi
th baseline HIV-1 RNA levels >30,000 copies/ml (RH, 0.20; 95% CI, 0.07-0.61
; p = .0040) were less likely to reach undetectable HIV-1 RNA levels than t
hose with baseline HIV-1 RNA levels <5000 copies/ml. Salvage highly active
antiretroviral therapy (HAART) strategies including saquinavir (SQV) at hig
h doses plus ritonavir (RTV) exert a significant long-term efficacy in more
than half of PI-experienced patients without significant additional toxici
ty. This therapeutic efficacy is strongly implemented by a switch at the lo
wer HIV-1 RNA levels.