F. Arenal et al., Evaluation of different PCR-based DNA fingerprinting techniques for assessing the genetic variability of isolates of the fungus Epicoccum nigrum, J APPL MICR, 87(6), 1999, pp. 898-906
Thirty-six strains of the fungus Epicoccum nigrum, isolated from different
substrata and ecosystems of Europe, America and Africa, were analysed using
1+ molecular markers included in 5 different genetic fingerprinting techni
ques: AP-PCR, tDNA-PCR, microsatellite-primed PCR, ARDRA and AFLP. All of t
he techniques used were able to differentiate the isolates, showing a high
genetic diversity within the species. However, the different techniques det
ected different levels of similarity among the strains; ARDRA shows the mos
t homogeneous results whilst AP-PCR shows the must heterogeneous. The simil
arity indices achieved for each strain were compared for the different tech
niques. The distribution obtained by microsatellite-primed PCR was similar
to those shown bq; AP-PCR techniques. tDNA-PCR and AFLP rendered similar di
stributions, and ARDRA showed remarkably different results from the other t
echniques. The results also reveal the lad; of an overall correlation betwe
en geographical or ecological origin of the isolates and their genotypes.