Growth competition between Candida dubliniensis and Candida albicans underbroth and biofilm growing conditions

Seven isolates each of Candida albicans and Candida dubliniensis were paire d (11 pairs) and examined for competitive interaction. Equal numbers of CFU of each competitor Here inoculated into Sabouraud dextrose broth and incub ated at 37 degrees C with vigorous shaking under conditions favorable to ei ther broth or biofilm growth. Surviving proportions of each competitor were calculated from the broth culture at 24 and 96 h and the biofilm culture a t 96 h, with species differentiation done on CHROMagar Candida medium. C. a lbicans had a competitive advantage ol er C. dubliniensis in broth culture and under biofilm growing conditions; however, with the presence of a suppo rting structure for biofilm formation, C. dubliniensis was able to better w ithstand the competitive pressures from C. albicans.