In vitro expression of pro alpha 1(I) collagen mRNA by human pre-osteoclastic cells

In vitro studies have demonstrated that the extracellular matrix modulates the cell phenotype. In the present study we have investigated in vitro pro alpha 1(1) collagen mRNA expression in a human pre-osteoclastic cell line ( FLG 29.1 cells) in basal condition and after various stimuli. In addition, in order to evaluate the effect of cell-cell interactions on collagen type I mRNA expression, we have cultured the human pre-osteoclastic cells FLG 29 .1 with either the human osteoblast-like cell line Saos-2 or the bovine bon e endothelial cell line BBE. We showed that the FLG 29.1 cells express pro alpha 1(1) collagen mRNA, whose expression is modulated by phorbol eaters ( TPA). Co-culturing FLG 29.1 cells with either Saos-2 or BBE cells induced d ecrease of pro alpha 1(1) collagen mRNA expression. (C)2000, Editrice Kurti s.