Rt. Schooley et al., Reduced mobilization of CD34(+) stem cells in advanced human immunodeficiency virus type 1 disease, J INFEC DIS, 181(1), 2000, pp. 148-157
Granulocyte colony-stimulating factor (r-met Hu G-CSF; filgrastim; 10 mu g/
kg/day for 7 days) was used to mobilize CD34(+) stem cells into the periphe
ral blood of human immunodeficiency virus type 1 (HIV-1)-infected individua
ls and a group of HIV-1-uninfected donors as a measure of immunologic reser
ve in HIV-1-infected people. G-CSF mobilized CD34(+) cells of HIV-1-infecte
d individuals with cell counts >500 CD4(+) cells/mm(3), as well as in HIV-1
-uninfected donors. In contrast, CD34(+) cell mobilization was significantl
y blunted in HIV-1-infected individuals with cell counts <500 CD4+ cells/mm
(3) (<200 cell days vs. >650 cell days, P < .0005, compared with the >500 C
D4(+) cell cohort). At least 1.75 X 10(7) CD34(+) cells were harvested by l
eukapheresis from patients in each study cohort. CD34(+) cell viability and
the ability to differentiate precursor cells into myeloid and erythroid pr
ogenitor cells were not affected by HIV-1 infection.