Echoviruses and Coxsackie B viruses that use human decay-accelerating factor (DAF) as a receptor do not bind the rodent analogues of DAF

Many serotypes of echovirus (EV) and Coxsackie B virus (CBV) bind human dec ay-accelerating factor (DAF) and use it as a receptor for infection, Analog ues for DAF have been isolated from mice and rats and characterized; these analogues have amino acid identities to human DAF of similar to 60%, EV ser otypes 3, 6', 7, 11-13, and 29 and CBV serotypes 1, 3, and 5 caused hemaggl utination of human erythrocytes but not rat or mouse erythrocytes, suggesti ng failure to bind rodent DAF. To confirm this evidence, radiolabeled virus es were incubated with transfected Chinese hamster ovary (CHO) cells that w ere abundantly expressing each type of DAF. Only cells that expressed human DAF bound virus, Although binding of EV and CBV was specific for human DAF , complement inhibition by DAF expressed in CHO cells was similar for each analogue.