Cell-cell adhesion molecules and signaling intermediates and their role inthe invasive potential of prostate cancer cells

Purpose: The highly variable natural history of prostate carcinoma may be r eflected in heterogeneity of invasive potential between tumors. Materials and Methods: We have examined two prostate cancer cell lines of l ow invasive potential (CAHPV10 and PZHPV7) and three cell lines of high inv asive potential (DU-145, PC-3 LNCapFGC), to determine whether specific adhe sion-molecule profiles correlated with their invasive behavior. Results: Using an in vitro invasion assay, we demonstrated that DU-145, LNC apFGC and PC-3 cells were highly invasive compared with CA-HPV-10 and PZ-HP V-7 cells. LNCapFGC cells expressed high levels of E-cadherin, alpha-, beta - and gamma-catenin, desmoglein, desmoplakin and GSK3 beta using immunoblot ting. This was, in general, comparable to immunohistochemical staining. PC- 3 cells had no E-cadherin or alpha-catenin, but expressed a high level of t he HGF/SF receptor c-Met. In contrast, DU-145 cells were found to express E -cadherin and low levels for all other protein molecules, except c-Met. The DU-145 cell line also lacked alpha-catenin expression. In CA-HPV-10 and PZ -HPV-7 cells, there was no detection of APC, PECAM-1, P-cadherin or Wnt-1. DU-145, LNCapFGC and PC-3 cells formed cell-cell aggregates, which were red uced by inclusion of anti-E-cadherin antibody and the motogen HGF/SF. Conclusion: These results show that prostate cancer cells exhibit a diverse expression of cell-cell adhesion molecules and their signaling intermediat es. The expression of these adhesion molecules bears an important relations hip with the invasive phenotype of these cells.