Ck. Chuang et al., Immunobiologic, cytogenetic and drug response features of a newly established cell line (SCRC-1) from renal small cell carcinoma, J UROL, 163(3), 2000, pp. 1016-1021
Purpose: We describe the establishment and preliminary characterization of
a cell line designated SCRC-1, which was derived from a primary renal small
cell carcinoma.
Materials and Methods: Continuous cultures of a primary stage IVa renal sma
ll cell carcinoma and a xenograft in nude mice derived therefrom were chara
cterized by immunohistology, electron microscopy, immunofluorescence/flow c
ytometry, cytogenetic analysis, and an in vitro drug resistance assay.
Results: SCRC-1 cells were reactive with antibodies to NSE, chromogranin-A,
bombesin, Bcl-2, CD44s, CD44v6, CD44v7 to 8, vimentin and S100 protein (pr
edominantly beta-subunit), and were unreactive with antibodies to EMA, CD54
, EGFR(R1), URO-5, URO-7, URO-8 and URO-10. A similar immunoprofile was als
o found in both the primary tumor and the xenograft. Cytogenetic analysis r
evealed the following common clonal aberrations in all 50 metaphases analyz
ed: 45, xx, t (X;10;18) (p11;p11;q11), -der(18)t(X;10;18), indicating the c
lonal nature of this neoplasm. SCRC-1 cells showed low drug resistance to c
yclophosphamide, doxorubicin, gemcitabine and fluorouracil, intermediate re
sistance to carmustine and mitomycin-C, and extreme resistance to cisplatin
.
Conclusion: We have documented the initial characterization of SCRC-1, whic
h may be the first cell line reported to be derived from a primary small ce
ll carcinoma of the kidney. This cell line can be used for further studies
uncovering the biology and histogenesis of this rare cancer and delineating
differences among small cell carcinomas of the kidney and other histologic
al types.