Insertion of autologous tendon grafts to the bone: a histological and immunohistochemical study of hamstring and patellar tendon grafts

This study examined the structure of the insertion of autologous tendon gra fts used for anterior cruciate ligament reconstruction. Biopsy specimens of the femoral and tibial bone graft interface were obtained at revision surg ery in 14 patients (6 with hamstring grafts, 8 with a patella tendon graft) . The specimens were analyzed by light microscopy and immunohistochemistry (confirming type I, type IT, and type III collagen). The insertions of hams tring autografts to the bone tunnel have three characteristic histological zones. Zone 1 is composed of the dense connective tissue of the graft. The collagen fibers of the graft enter the bone under oblique angles. Zone 2 is composed of woven bone with a sharp transition to the lamellar bone of the tibia (zone 3). Immunohistochemistry revealed the presence of type I and t ype In collagen within the dense connective tissue of the graft. The woven and lamellar bone showed positive immunostaining for antibodies against typ e I collagen only. This structure resembled a fibrous ligament or tendon in sertion. In the majority of patients with a patella tendon graft the struct ure of the insertion resembled a chondral enthesis. The chondral insertion of the graft to the bone is composed of four characteristic zones. Between the dense connective tissue of the graft (zone 1) and bone (zone 4) there i s a zone of fibrocartilage (zone 2). Close to the bone the fibrocartilage i s mineralized (zone 3). Within the fibrocartilage the immunohistochemical a nalysis confirmed type LI collagen. This structure resembled the chondral e nthesis of the nom-tal anterior cruciate ligament. However, in cases in whi ch the distal bone bloc has been fixated outside the tibial tunnel, the tib ial insertion of the patellar tendon graft resembled a fibrous insertion. W hile both types of tendon grafts heal to the bone of femur and tibia, the i nsertion of patella tendon grafts healing by bone plug incorporation resemb les the chondral insertion of the normal anterior cruciate ligament and may have a more physiological connection to the bone than hamstring grafts.