Age-related changes of gene expression in mouse kidney: fluorescence differential display - PCR analyses

We used a fluorescence differential display - PCR (FDD-PCR) technique to an alyze the genes expressed in mouse kidneys collected at nine different deve lopmental stages ranging from 3 days to 15 months after birth. We found ten genes that were age-dependent and differentially-expressed in the kidneys during our experimental period. We confirmed by comparative RT-PCR that of the ten cDNAs, seven showed reproducible age-dependent expression. Four of the nucleotide sequences of these cDNA clones, had high homology with known genes (fibronectin, soluble guanylyl cyclase alpha-1 subunit, cytosolic al dehyde dehydrogenase and mitochondrial DNA), and three with expressed seque nce tags of unknown genes. The FDD-PCR method was very useful for detecting new age-related genes expressed differentially in the mouse kidney. (C) 20 00 Elsevier Science Ireland Ltd. All rights reserved.