Purpose: The theoretical velocity associated with (V) over dot O-2max (v(V)
over dot O-2max) defined by Daniels (1985) is extrapolated from the submax
imal (V) over dot O-2-velocity relationship. (V) over dot O-2 is generally
determined by assuming that the aerobic response reacts like a linear first
-order system at the beginning of square-wave exercise, with a steady-state
reached by the 4th minute. However, at supra-ventilatory threshold work ra
tes, the steady state in (V) over dot O-2 is delayed or not attained. Metho
ds: The present study was carried out to compare three values for v(V) over
dot O-2max, determined with Daniels' method, but with (V) over dot O-2 eit
her measured at the 4th minute (v(V) over dot O-2max4), the 6th minute (v(V
) over dot O-2max6), or after the attainment of the true steady-state (v(V)
over dot (2maxSS)). The metabolic response during square-wave exercise at
each of the three v(V) over dot O-2max were also assessed. Results: These v
elocities were significantly different (P < 0.05), but v(V) over dot O-2max
SS and v(V) over dot O-2max6 were highly correlated (r =0.98; P < 0.05). Bl
ood lactate concentrations measured after exercise at velocities very close
to the three v(V) over dot O-2max were similar and the end-exercise (V) ov
er dot O-2 were not different from (V) over dot (2max), but the time requir
ed to elicit 95% (V) over dot O-2max during these three square-wave rests w
ere significantly different. Conclusion: Therefore, when v(V) over dot O-2m
ax is determined by extrapolation from the submaximal (V) over dot O-2-velo
city relationships, submaximal (V) over dot O-2 should be measured beyond t
he 6th minute of square-wave exercise (at least if it takes 30 s to reach t
he desired velocity) to ensure that all v(V) over dot O-2max reported in fu
ture studies describe a similar quantitative index.