Growth inhibition of a human ovarian tumor by a novel paclitaxel derivative in SCID mice

We report here the toxicity and therapeutic effects of 2'-alpha-bromohexade tanoyl paclitaxel (BrC16HT), a prodrug form of paclitaxel, in mice. Paclita xel is the active ingredient of Taxol(R). The maximum tolerated dose, at a one dose per day far 5-day schedule, was 37.5 mg/kg for BrC16HT compared to 12.5 for Taxol(R) administered IP, and was 12.5-25 mg/kg for either agent administered IV. Dose-dependent therapeutic effects were found for BrC16HT against a human ovarian tumor (OVCAR-3) grown in SCID mice. IP treatments w ith BrC16HT against early or established IP-implanted OVCAR-3 tumor increas ed mean survival times more than treatment with Taxol(R). Long-term survivo rs were found only in groups treated with BrC16HT. Intravenously administer ed BrC16HT was more effective than Taxol(R) against SC OVCAR-3 tumor. Early treatment (25 mg/kg x 5) completely inhibited tumor growth through 120 day s after tumor implantation. Pharmacokinetic studies suggest that BrC16HT is slowly hydrolyzed to paclitaxel and circulates longer than paclitaxel from Taxol(R). Thus, BrC16HT may provide sustained levels of paclitaxel, which may contribute to the increased efficacy of BrC16HT compared to Taxol(R).