Conformational dynamics of calcium green 1 by fluorescence correlation spectroscopy

Authors
Tessier, A Meallet-Renault, R Denjean, P Miller, D Pansu, RB
Citation
A. Tessier et al., Conformational dynamics of calcium green 1 by fluorescence correlation spectroscopy, PCCP PHYS C, 1(24), 1999, pp. 5767-5769
Citations number
14
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
PCCP PHYSICAL CHEMISTRY CHEMICAL PHYSICS
ISSN journal
14639076 → ACNP
Volume
1
Issue
24
Year of publication
1999
Pages
5767 - 5769
Database
ISI
SICI code
1463-9076(199912)1:24<5767:CDOCG1>2.0.ZU;2-H
Abstract
Fluorescence correlation spectroscopy was used to characterise the conforma tional equilibrium in "Calcium Green 1", a fluorescent indicator of calcium . In the calcium bound form, a triplet bunching time of 6.7 mu s is seen, W e show that, in the absence of calcium, 10% of the indicator is in a fluore scent conformation that inter-converts to a non-fluorescent form in 10 ps. This confirms the probing mechanism based on conformation controlled PET pr oposed for Calcium Green 1.