Am. Nuutila et al., Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.), PLANT SCI, 151(1), 2000, pp. 85-92
In recent years particle bombardment has become the most used method for ge
ne transfer to barley (Hordeum vulgare L.). Transformation efficiency depen
ds greatly on the ability of the target material to regenerate into green p
lants. In this work we improved the regeneration efficiency of polyembryoge
nic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper c
oncentrations in the media. (C) 2000 Published by Elsevier Science Ireland
Ltd. All rights reserved.