A COMPARISON OF MONOCLONAL-ANTIBODY IMMOBILIZATION OF PLATELET ANTIGEN (MAIPA) AND IMMUNOBEAD METHODS FOR DETECTION OF GPIIB IIIA ANTIPLATELET ANTIBODIES IN IMMUNE THROMBOCYTOPENIC PURPURA/

Authors
CROSSLEY A CALVERT JE TAYLOR PRA DICKINSON AM
Citation
A. Crossley et al., A COMPARISON OF MONOCLONAL-ANTIBODY IMMOBILIZATION OF PLATELET ANTIGEN (MAIPA) AND IMMUNOBEAD METHODS FOR DETECTION OF GPIIB IIIA ANTIPLATELET ANTIBODIES IN IMMUNE THROMBOCYTOPENIC PURPURA/, TRANSFUSION MEDICINE, 7(2), 1997, pp. 127-134
Citations number
18
Categorie Soggetti
Hematology
Journal title
TRANSFUSION MEDICINE → ACNP
ISSN journal
09587578
Volume
7
Issue
2
Year of publication
1997
Pages
127 - 134
Database
ISI
SICI code
0958-7578(1997)7:2<127:ACOMIO>2.0.ZU;2-K
Abstract
In this study we have compared two assays for the detection of autoant ibodies GpIIb/IIIa, platelet bound and in serum, in immune thrombocyto penic purpura (ITP). Both assays were found to have a similar sensitiv ity, but the monoclonal antibody immobilization of platelet antigen (M AIPA) assay was more reproducible than the immunobead assay. The MAIPA and immunobead assay demonstrated an 81% concordance of results for s erum antibody detection and a 78% concordance for platelet-associated antibody detection, with an 8-12% incidence of false positive or negat ive results.