Large-scale monitoring of host cell gene expression during HIV-1 infectionusing cDNA microarrays

Human immunodeficiency virus type 1 (HIV-1) infection alters the expression of host cell genes at both the mRNA and protein levels. To obtain a more c omprehensive view of the global effects of HIV infection of CD4-positive T- cells at the mRNA level, we performed cDNA microarray analysis on approxima tely 1500 cellular cDNAs at 2 and 3 days postinfection (p.i.) with HIV-1. H ost cell gene expression changed little at 2 days p.i,, but at 3 days p.i. 20 cellular genes were identified as differentially expressed. Genes involv ed in T-cell signaling, subcellular trafficking, and transcriptional regula tion, as well as several uncharacterized genes, were among those whose mRNA s were differentially regulated. These results support the hypothesis that HIV-1 infection alters expression of a broad array of cellular genes and pr ovides a framework for future functional studies on the differentially expr essed mRNA products. (C) 2000 Academic Press.