Partial antigenic characterization of different potato virus YNTN strain isolates

Eight isolates of potato virus Y NTN strain (PVY-NTN) of different origin w ere studied by means of monoclonal antibodies (MAbs) in non-competitive and competitive enzyme-linked immunosorbent assay (ELISA), and by immunoblot a nalysis of the viral coat protein (CP). As the MAbs reacted with the denatu red viral CP, their epitopes must be continuous. The ELISA data demonstrate that the epitopes are topologically different. The epitopes may be located on the N-terminal part of CP as showed its partial amino acid sequencing a nd the immunoblot analysis.