The binding of amino acids to the herbicide 2,4-dichlorophenoxy acetic acid

The interaction of amino acids with the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) was studied by charge-transfer chromatography carried out on diatomaceous layers covered with different amount of: 2,4-D and the effect of salts on the strength of interaction was elucidated. It: was established that Arg, His, Lys, Orn, Phe and Trp binds to 2,4-D, the binding process i s of saturation character. Principal component analysis proved that the con centration of 2,4-D exerts the highest impact on the interaction and the ef fect of salts is of secondary importance. The results suggest that these am ino acid residues may account for the binding of 2,4-D to proteins and can play a considerable role in the detoxification processes by forming conjuga tes with 2,4-D.