Application of Convective Interaction Media disks with immobilised glucoseoxidase for on-line glucose measurements

A new polymeric macroporous material, Convective Interaction Media (CIM) wa s applied as a support for glucose oxidase (GOD) immobilisation. CIM epoxy disks with the immobilised enzyme were integrated as an enzyme reactor in a flow injection analysis (FIA) system and applied to on-line monitoring of glucose during cultivation of Saccharomyces cerevisiae and citric acid prod uction by Aspergillus niger. The developed CIM GOD disk-FIA system exhibite d good signal reproducibility and satisfactory long-term stability with a l inear response in the range 10-200 mg 1(-1). The CIM disk applied as an enz yme reactor proved to be a successful replacement for conventionally used p acked-bed columns and as such it was well suited for on-line monitoring of bioprocesses. (C)2000 Elsevier Science B.V. All rights reserved.