Voltammetry of the erythromycin-modified glassy carbon electrode and interaction with DNA

The chemically modified electrode (CME) which was constructed by covalently attaching Erythromycin (ERM) to a glassy carbon (GC) surface was investiga ted in Tris-HCl buffer (pH=6.0) by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In the potential range -0.5 to +0.4V, CME yields a pair of stable redox waves. It is the carbonyl group of the ERM molecule immobilized on the GC surface that behaves as a mio-electron redox process involving one proton. The interaction of CME with DNA was also studied by D PV. The CME shows the same interaction with DNA as that in the solution. An d the interactive model between ERM and DNA was proved by fluorescence in a queous solutions.