Vaccination of Atlantic halibut Hippoglossus hippoglossus L., and spotted wolffish Anarhichas minor L., against atypical Aeromonas salmonicida

Atypical furunculosis caused by atypical Aeromonas salmonicida is an increa sing problem in commercial halibut farming, and a potential problem in farm ing of spotted wolffish in Norway. Halibut, Hippoglossus hippoglossus L., a nd spotted wolffish, Anarhichas minor L., vaccinated with oil-emulsified va ccines against atypical furunculosis, demonstrated a relative percent survi val (RPS) of approximately 90 when challenged with homologous isolates. Bat h and aqueous injection vaccines failed to protect against disease when cha llenged 10 weeks post-vaccination. High antibody titres were produced in bo th species after vaccination with oil-emulsified vaccines, and the major an tibody response were against A-layer, LPS and some minor outer membrane (OM ) proteins. (C) 2000 Elsevier Science B.V. All rights reserved.