Testing wound-healing activity in T15 fibroblast cultures: A morphometric analysis

The purpose was to evaluate the use of mouse T15 fibroblast cell cultures f or the investigation of wound-healing activity. In order to investigate the ir mechanisms of action, the effects of drugs with wound-healing activities were compared by using morphometric analyses by microscopy after cell stai ning. A number of parameters were used to evaluate the effects of titrated extracts from Centella asiatica and dexpanthenol (drugs that have been used in medical practice for their wound-healing activities) on cultured mouse T15 fibroblasts. These parameters were: the total number of cells; the numb er of T15 cells in mitosis; the percentages of fusiform, polygonal, round a nd vacuole-containing cells; and the number of intracellular collagen granu les. The results indicate that these two drugs exhibit wound-healing activi ties by activating fibroblast cells, and have cytoprotective effects, altho ugh their mechanisms of action on mouse T15 fibroblasts were different. On the basis of our findings, mouse T15 fibroblast cell cultures seem to be us eful for the pharmacological screening of compounds with wound-healing acti vity.