The gene for human TATA-binding-protein-associated factor (TAF(II)) 170: structure, promoter and chromosomal localization

Citation
Ja. Van Der Knaap et al., The gene for human TATA-binding-protein-associated factor (TAF(II)) 170: structure, promoter and chromosomal localization, BIOCHEM J, 345, 2000, pp. 521-527
Citations number
36
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL JOURNAL
ISSN journal
02646021 → ACNP
Volume
345
Year of publication
2000
Part
3
Pages
521 - 527
Database
ISI
SICI code
0264-6021(20000201)345:<521:TGFHTF>2.0.ZU;2-5
Abstract
The TATA-binding protein (TBP) plays a central role in eukaryotic transcrip tion and forms protein complexes with TBP-associated factors (TAFs). The ge nes encoding TAF(II) proteins frequently map to chromosomal regions altered in human neoplasias. TAF(II)170 of B-TFIID is a member of the SF2 superfam ily of putative helicases. Members of this superfamily have also been impli cated in several human genetic disorders. In this study we have isolated hu man genomic clones encoding TAF(II)170 and we show that the gene contains 3 7 introns. Ribonuclease-protection experiments revealed that TAF(II)170 has multiple transcription start sites, consistent with the observation that t he promoter lacks a canonical TATA box and initiator element. Deletion anal ysis of the promoter region showed that a fragment of 264 bp is sufficient to direct transcription. In addition, we determined the chromosomal localiz ation by two independent methods which mapped the gene to human chromosome 10q22-q23 between the markers D10S185 and WI-1183. The region surrounding t hese markers has been implicated in several human disorders.