Ja. Van Der Knaap et al., The gene for human TATA-binding-protein-associated factor (TAF(II)) 170: structure, promoter and chromosomal localization, BIOCHEM J, 345, 2000, pp. 521-527
The TATA-binding protein (TBP) plays a central role in eukaryotic transcrip
tion and forms protein complexes with TBP-associated factors (TAFs). The ge
nes encoding TAF(II) proteins frequently map to chromosomal regions altered
in human neoplasias. TAF(II)170 of B-TFIID is a member of the SF2 superfam
ily of putative helicases. Members of this superfamily have also been impli
cated in several human genetic disorders. In this study we have isolated hu
man genomic clones encoding TAF(II)170 and we show that the gene contains 3
7 introns. Ribonuclease-protection experiments revealed that TAF(II)170 has
multiple transcription start sites, consistent with the observation that t
he promoter lacks a canonical TATA box and initiator element. Deletion anal
ysis of the promoter region showed that a fragment of 264 bp is sufficient
to direct transcription. In addition, we determined the chromosomal localiz
ation by two independent methods which mapped the gene to human chromosome
10q22-q23 between the markers D10S185 and WI-1183. The region surrounding t
hese markers has been implicated in several human disorders.