Purification of porcine plasma factor VIII using chromatographic methods

Factor VIII was purified from porcine plasma using adsorption on aluminium hydroxide with CM-cellulose as a filtration aid, cold ethanol precipitation , and two anion-exchange (Q-Sepharose fast flow) chromatographies. The fina l product was purified 264-fold and had a specific activity of 10 U mg(-1). The method is suitable to produce purified porcine FVIII by an easy proces s where all steps can be scaled up. The final product is free of von Willeb rand factor that is responsible for the main side effects in patients. Fina lly, this method can be used to obtain purified porcine plasma FVIII for us e in haemophilic patients with inhibitors.