Irreversible lysozyme inactivation and aggregation induced by stirring: kinetic study and aggregates characterisation

Authors
Colombie, S Gaunand, A Rinaudo, M Lindet, B
Citation
S. Colombie et al., Irreversible lysozyme inactivation and aggregation induced by stirring: kinetic study and aggregates characterisation, BIOTECH LET, 22(4), 2000, pp. 277-283
Citations number
14
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
4
Year of publication
2000
Pages
277 - 283
Database
ISI
SICI code
0141-5492(200002)22:4<277:ILIAAI>2.0.ZU;2-N
Abstract
Stirring strongly enhanced irreversible inactivation and aggregation of lys ozyme being studied as a model enzyme. From 0 to 740 rpm (equivalent to imp eller tip speeds from 0 to 0.77 m s(-1)), the inactivation kinetic constant was proportional to the power imparted by the impeller. Collisions between inactive and native molecules induced inactivation of the latter and led t o lysozyme aggregation. These fractal aggregates of lysozyme were made of m onomers, dimers and trimers.