Syntheses of hydroxybenzyl-alpha-glucosides by amyloglucosidase-catalyzed transglycosylation

Glycosides were synthesized from soluble starch and hydroxybenzylalcohols ( o, m and p) by the transglycosylation activity of amyloglucosidase from Rhi zopus sp. in a water system. The synthesized glycosides were identified as hydroxybenzyl-alpha-glucosides, of which one molecule of hydroxybenzylalcoh ol was bound to 1-OH of glucose. The transglycosylation reaction was perfor med in a reaction system containing 50 mg starch, 50 mg hydroxybenzylalcoho l, and 10 units of enzyme in pH 5.0 at 45 degrees C. The glycoside was rapi dly hydrolyzed to glucose and hydroxybenzylalcohol by the alpha-glucosidase .