A method for the detection and confirmation of heavy metal toxicants in sed
iment elutriates based on a urease inhibition assay, ICP-AES analysis and E
DTA chelation in the frame of toxicity identification evaluation (TIE) is p
resented. Zinc was identified as the major toxicant in pH(stat) elutriates
of sediments of the river Saale (Germany). Implications of natural and anth
ropogenic chelating agents, which are frequently present in environmental s
amples, on toxicity confirmation of heavy metals based on the toxic unit ap
proach are discussed. (C) 2000 Elsevier Science Ltd. All rights reserved.