Gliclazide reduces the induction of human monocyte adhesion to endothelialcells by glycated albumin

Objective: To examine the kinetic of human monocyte adhesion to endothelial cells stimulated by glycated albumin, the contributive role of cell adhesi on molecules to this effect, and the effect of gliclazide-an hypoglycemic d rug with antioxidant properties-on these parameters. Methods: In-vitro experiments performed in the presence and absence of glic lazide consisted of: (1) time-dependent determination of human monocyte adh esion to human endothelial cells (ECs) pre-exposed to glycated albumin; (2) evaluation of adhesion after incubation of ECs with antibodies against cel l surface adhesion molecules; and (3) determination of EC surface adhesion molecules and of the activity of the transcription factor NF-kappa B. Results: Exposition of human ECs for 1-48 h to 100 mu g/ml glycated albumin led to a time-dependent increase in human monocyte adhesion to endothelium . Pretreatment of ECs with 10 mu g/ml gliclazide significantly decreased th e glycated albumin-stimulated monocyte adhesion to these cells. Anti-antibo dies against E-selectin (ELAM-1), vascular cell adhesion molecule-1 (VCAM-1 ) and intercellular adhesion molecule-1 (ICAM-1) also reduced the stimulato ry effect of glycated albumin on monocyte adhesion. In glycated albumin-tre ated ECs, an induction of both soluble and cell associated expression of EL AM-1, VCAM-1 and ICAM-1, an augmentation in the levels of these molecule tr anscripts and an increase in the DNA binding activity for NF-kappa B in the promoters of these antigens were observed. Gliclazide markedly inhibited t he induction of all these parameters. Conclusions: Glycated albumin stimulates human monocyte adhesion to ECs by inducing cell associated ELAM-1, ICAM-1 and VCAM-1. Gliclazide effectively inhibits monocyte adhesion to ECs by reducing glycated albumin induction of EC adhesion molecules and NF-kappa B activation. These results suggest tha t gliclazide may be beneficial in the prevention of endothelial disturbance s associated with hyperglycemia in diabetic patients.