MODELING OF ESCHERICHIA-COLI FERMENTATIONS - COMPARISON OF MULTICOMPARTMENT AND VARIABLE-STRUCTURE MODELS

Two novel approaches for modelling processes that can be described by a sequence of phases (metabolic states) are suggested and applied to E scherichia Coli fermentations. The first approach uses a multi-compart ment model framework, coupled with knowledge-based logic. In the secon d approach the multi-compartment model is reduced into the Variable St ructure Model consisting of a battery of alternative submodels, each o f which qualitatively represents one of the process steps. Furthermore , simulated intracellular process variables are compared with the outp ut of a multi-wavelength fluorosensor and excitation-emission pairs th at predict best these variables are identified.